Electrostatic Basis of Anion Over Cation Selectivity in the CFTR Chloride Channel

Locating the Anion-selectivity Filter of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Chloride Channel

The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel; the site and mechanism of charge selectivity is unknown. We previously reported that cysteines substituted, one at a time, for Ile331, Leu333, Arg334, Lys335, Phe337, Ser341, Ile344, Arg347, Thr351, Arg352, and Gln353, in and flanking the sixth membrane-spanning segment (M6), reacted with charged, sulfhydr...

Permeation through the CFTR chloride channel.

The cystic fibrosis transmembrane conductance regulator (CFTR) protein forms a Cl(-) channel found in the plasma membranes of many epithelial cells, including those of the kidney, gut and conducting airways. Mutation of the gene encoding CFTR is the primary defect in cystic fibrosis, a disease that affects approximately 30 000 individuals in the United States alone. Alteration of CFTR function ...

CFTR, a rectifying, non-rectifying anion channel?

Pharmacology of CFTR chloride channel activity.

Pharmacology of CFTR Chloride Channel Activity. Physiol. Rev. 79, Suppl.: S109-S144, 1999. - The pharmacology of cystic fibrosis transmembrane conductance regulator (CFTR) is at an early stage of development. Here we attempt to review the status of those compounds that modulate the Cl- channel activity of CFTR. Three classes of compounds, the sulfonylureas, the disulfonic stilbenes, and the ary...

Anion and Cation Permeability of a Chloride Channel in Rat Hippocampal Neurons

I N T R O D U C T I O N This article examines the ionic selectivity o f an anion channel that is consistently found in the somatic membran e o f hippocampal neurons f rom embryonic rats. Characteristic o f the channel is a relatively small unitary conductance (~30 pS in symmetrical 150 mM NaCI saline) that is tu rned on and of f by a weakly voltage-dependent , kinetically complex gating process...